RFLP mapping of thymidine kinase mutants places Dl7S4 proximal to the human TK1 locus
نویسندگان
چکیده
منابع مشابه
StuI RFLP in the human ABL locus.
Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...
متن کاملHuman Thymidine Kinase
Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that t...
متن کاملDiverse herpes simplex virus type 1 thymidine kinase mutants in individual human neurons and Ganglia.
Mutations in the thymidine kinase gene (tk) of herpes simplex virus type 1 (HSV-1) explain most cases of virus resistance to acyclovir (ACV) treatment. Mucocutaneous lesions of patients with ACV resistance contain mixed populations of tk mutant and wild-type virus. However, it is unknown whether human ganglia also contain mixed populations since the replication of HSV tk mutants in animal neuro...
متن کاملTransfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.
Transformation of human cells from a thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75)-negative to a thymidine kinase-positive phenotype has been achieved by using purified DNA from herpes simplex virus type 2. The specific activity of the DNA was in the range 0.5 to 2.0 transformants per microng and the efficiency of gene transfer was up to 1 transformant per 10(5) recipient ...
متن کاملClinical significance of serum thymidine kinase 1 (TK1) expression in patients with non-small cell lung cancer
Thymidine kinase 1 (TK1) is an enzyme involved in nucleic acid synthesis and is therefore considered to be an important marker of tumor proliferation. In this study, the use of serum thymidine kinase 1 expression for the diagnosis, treatment, and prognosis of patients with non-small cell lung cancer (NSCLC) following chemotherapy was investigated. The TK1 values of NSCLC patients were determine...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.13.3748